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Fig. 4 | Cancer Cell International

Fig. 4

From: Circular RNA hsa_circ_0061395 accelerates hepatocellular carcinoma progression via regulation of the miR-877-5p/PIK3R3 axis

Fig. 4

Circ_0061395 served as a sponge for miR-877-5p in HCC cells. a The complementary binding sites between circ_0061395 and miR-877-5p were predicted by the CircInteractome database. b Dual luciferase reporter assay was performed to assess the luciferase activity in SNU-387 and Huh7 cells cotransfected with luciferase plasmids containing MUT-circ_0061395 or WT-circ_0061395 and miRNA NC or miR-877-5p mimic. c After RIP assay, the levels of circ_0061395 and miR-877-5p were examined by qRT-PCR. df The expression of miR-877-5p in HCC serum, tissues, and cells was detected by qRT-PCR. g After inhibitor NC or miR-877-5p inhibitor transfection, the expression of miR-877-5p in SNU-387 and Huh7 was examined by qRT-PCR. (H) The expression of miR-877-5p in SNU-387 and Huh7 cells transfected with si-NC, si-circ_0061395, si-circ_0061395 + inhibitor NC, or si-circ_0061395 + miR-877-5p inhibitor was analyzed by qRT-PCR. *P < 0.05

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