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Fig. 6 | Cancer Cell International

Fig. 6

From: Circular RNA hsa_circ_0061395 accelerates hepatocellular carcinoma progression via regulation of the miR-877-5p/PIK3R3 axis

Fig. 6

Circ_0061395 regulated PIK3R3 expression through sponging miR-877-5p in HCC cells. a The binding sites of PIK3R3 in miR-877-5p were predicted by the starBase database. b Dual-luciferase reporter assay was executed to determine the binding sites between PIK3R3 and miR-877-5p in SNU-387 and Huh7 cells. cg QRT-PCR and western blotting were employed to assess the levels of PIK3R3 mRNA and/or protein in HCC serum, tissues, and cells. h After pc-NC or pc-PIK3R3 transfection, the level of PIK3R3 protein in SNU-387 and Huh7 cells was detected with western blotting. (I-N) SNU-387 and Huh7 cells were transfected with miRNA NC, miR-877-5p mimic, miR-877-5p mimic + pc-NC, or miR-877-5p mimic + pc-PIK3R3. i The level of PIK3R3 protein in SNU-387 and Huh7 cells was analyzed by western blotting. jn The viability, colony formation, invasion, migration, cell cycle progression, and apoptosis of SNU-387 and Huh7 cells were analyzed by CCK-8, plate clone, transwell, or flow cytometry assays. o The level of PIK3R3 protein in SNU-387 and Huh7 cells transfected with si-NC, si-circ_0061395, si-circ_0061395 + inhibitor NC, or si-circ_0061395 + miR-877-5p inhibitor was measured by western blotting. *P < 0.05

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