Fig. 2
From: LncRNA PCAT6 regulates the progression of pituitary adenomas by regulating the miR-139-3p/BRD4 axis
PCAT6 was mainly located in the cytoplasm, and it regulated cell viability, migration, invasion, proliferation, and miR-139-3p expression in PA cells. a, b The transfection efficiencies of shPCAT6 in RC-4B/C and GH3 cells were determined by RT-qPCR. GAPDH was used as an internal control. c, d The expression location of PCAT6 in RC-4B/C and GH3 cells were determined by RT-qPCR. GAPDH and U6 were used as internal controls. e, f The expressions of miR-139-3p in RC-4B/C and GH3 cells after transfection were detected by RT-qPCR. U6 was used as an internal control. g, h The viability of RC-4B/C and GH3 cells after transfection was detected by CCK-8 assay. i, l–m The migration of RC-4B/C and GH3 cells after transfection was detected by wound healing assay (Magnification × 100). j, n, o The invasion of RC-4B/C and GH3 cells after transfection was detected by transwell assay (Magnification × 250). k, p, q The proliferation of RC-4B/C and GH3 cells after transfection was detected by colony formation assay. (***P < 0.001, vs. shNC; ###P < 0.001, vs. nuclear). shNC negative control of shRNA