Fig. 4

CircPIP5K1A promoted the expression of the TCF12 and PI3K/AKT signaling pathways. a TCF12 expression in glioma and adjacent normal tissues was detected by RT-PCR. b Protein expression of TCF12 in five cases of glioma and adjacent normal tissues was detected by western blot. c GEPIA (http://gepia.cancer-pku.cn/) was used for analyzing TCF12 expression in GBM and LGG. d TCF12 in glioma tissues and normal cerebral tissues was detected by IHC (data from The Human Protein Atlas (https://www.proteinatlas.org/). e Person correlation analysis detected the correlation between circPIP5K1A and TCF12 in the glioma tissues. f, k–m assay was used to analyze the relationship between TCF12 expression with glioma prognosis. g, h RT-PCR was performed to determine the expression of TCF12 mRNA (g) and protein (h). i GEPIA database showed a positive correlation between TCF12 and AKT1 in LGG. j Western blot was conducted to detect the PI3K/AKT activation. *P < 0.05, **P < 0.01, ***P < 0.001