Fig. 5

Overexpressing TCF12 promoted the malignant phenotypes of glioma cells. a Overexpression and knockdown models of TCF12 were constructed in U87 and A172, respectively, and the TCF12 expression was detected by western blot. B-C: CCK8 (b) and BrdU assay (c) were employed to detect cell proliferation. e–g Flow cytometry and western blot were used to verify the effect of TCF12 regulation on apoptosis. g Transwell assay was conducted to examine the effect of TCF12 regulation on cell invasion. h Western blot was carried out to measure the effect of TCF12 regulation on EMT. i The effect of TCF12 on PI3K/AKT was determined by western blot. *P < 0.05, **P < 0.01, ***P < 0.001