Fig. 12

ETV4 promoted EGFR transcription to activate ERK signaling pathway. a Expression of EGFR mRNA and protein in cells detected by RT-qPCR and Western blot. b The binding of ETV4 to EGFR promoter in HEK293 and HCT116 cells detected by Luciferase assay. c The binding of ETV4 to EGFR promoter by CHIP. *p < 0.05 compared with the NC group, MUT, or IgG. Each experiment was repeated 3 times independently. Data between the two groups were compared by unpaired t-test