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Fig. 7 | Cancer Cell International

Fig. 7

From: Overexpression of microRNA-29b inhibits epithelial-mesenchymal transition and angiogenesis of colorectal cancer through the ETV4/ERK/EGFR axis

Fig. 7

miR-29b reduces expression of ETV4 and inhibits the activation of the ERK signaling pathway. a miR-29b expression (normalized to U6) and mRNA expression (normalized to GAPDH) in HCT116 cells transfected with miR-29b mimic, miR-29b inhibitor and/or siRNA-ETV4 determined using RT-qPCR. b Western blots and quantitative analysis of ETV4, phosphorylated ERK1/2, ERK1/2, MMP-2, MMP-9, Vimentin, and VEGF proteins (normalized to GAPDH) in HCT116 cells transfected with miR-29b mimic, miR-29b inhibitor and/or siRNA-ETV4. *p < 0.05 compared with the blank and NC groups. #p < 0.05 compared with the DMSO group. Each experiment was repeated 3 times independently. Data among multiple groups were analyzed by one-way ANOVA, followed by a Tukey multiple comparisons posttest

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