Table 1 Primer sequences for RT-qPCR
Gene | Primer sequence |
|---|---|
miR-29b | F: 5′-TGAACCTTTGTCTGGGCAACT-3’ |
R: 5′-TGGTATCCTTGAGGGATTGGTTC-3′ | |
ETV4 | F: 5′-CACTCCCCTACCACCATGGA-3′ |
R:5′-GGACTTGATGGCGATTTGTC-3′ | |
ERK1 | F: 5′-CAGGAGACAGCACGCTTCCAG-3′ |
R: 5′-TCTAACAGTCTGGCGGGAGAGG-3′ | |
ERK2 | F: 5′-GCTGTTCCCAAATGCTGACT-3′ |
R: 5′-CTCGTCACTCGGGTCGTAAT-3′ | |
MMP-2 | F: 5′-AGTTTCCATTCCGCTTCCAG-3′ |
R: 5′-CGGTCGTAGTCCTCAGTGGT-3′ | |
MMP-9 | F: 5′-GAAGATGCTGCTGTTCAG-3′ |
R: 5′-AAATAGGCTTTCTCTCGGTA-3′ | |
E-cadherin | F: 5′-GACCAAGTGACCACCTTA-3′ |
R: 5′-AGAGCAGCAGAATCAGAAT-3′ | |
Vimentin | F: 5′-CATTGAGATTGCCACCTAC-3′ |
R: 5′-TCGTTGATAACCTGTCCAT-3′ | |
VEGF | F: 5′-CCATGAACTTTCTGCTCTCTTG-3′ |
R: 5′-GGTGAGAGGTCTAGTTCCCGAA-3′ | |
TSP-1 | F: 5′-GTGTTTGACATCTTTGAACTC-3′ |
R: 5′-CCAAAGACAAACCTCACATTC-3′ | |
GAPDH | F: 5′-GGTATCGTGGAAGGACTC-3′ |
R: 5′-GGGATGATGTTCTGGAGAG-3′ | |
U6 | F: 5′-GTGCTCGCTTCGGCAGCACATATAC-3′ |
R: 5′-AAAAATATGGAACGCTTCACGAATTTG-3′ |