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Table 1 Primer sequences for RT-qPCR

From: Overexpression of microRNA-29b inhibits epithelial-mesenchymal transition and angiogenesis of colorectal cancer through the ETV4/ERK/EGFR axis

Gene Primer sequence
miR-29b F: 5′-TGAACCTTTGTCTGGGCAACT-3’
R: 5′-TGGTATCCTTGAGGGATTGGTTC-3′
ETV4 F: 5′-CACTCCCCTACCACCATGGA-3′
R:5′-GGACTTGATGGCGATTTGTC-3′
ERK1 F: 5′-CAGGAGACAGCACGCTTCCAG-3′
R: 5′-TCTAACAGTCTGGCGGGAGAGG-3′
ERK2 F: 5′-GCTGTTCCCAAATGCTGACT-3′
R: 5′-CTCGTCACTCGGGTCGTAAT-3′
MMP-2 F: 5′-AGTTTCCATTCCGCTTCCAG-3′
R: 5′-CGGTCGTAGTCCTCAGTGGT-3′
MMP-9 F: 5′-GAAGATGCTGCTGTTCAG-3′
R: 5′-AAATAGGCTTTCTCTCGGTA-3′
E-cadherin F: 5′-GACCAAGTGACCACCTTA-3′
R: 5′-AGAGCAGCAGAATCAGAAT-3′
Vimentin F: 5′-CATTGAGATTGCCACCTAC-3′
R: 5′-TCGTTGATAACCTGTCCAT-3′
VEGF F: 5′-CCATGAACTTTCTGCTCTCTTG-3′
R: 5′-GGTGAGAGGTCTAGTTCCCGAA-3′
TSP-1 F: 5′-GTGTTTGACATCTTTGAACTC-3′
R: 5′-CCAAAGACAAACCTCACATTC-3′
GAPDH F: 5′-GGTATCGTGGAAGGACTC-3′
R: 5′-GGGATGATGTTCTGGAGAG-3′
U6 F: 5′-GTGCTCGCTTCGGCAGCACATATAC-3′
R: 5′-AAAAATATGGAACGCTTCACGAATTTG-3′
  1. miR-29b, microRNA-29b; RT-qPCR, reverse transcription quantitative polymerase chain reaction; ETV4, ETS variant 4; ERK, extracellular regulated protein kinases; MMP, matrix metalloproteinase; VEGF, vascular endothelial growth factor; TSP-1, thrombospondin 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward; R, reverse