Fig. 2
Silencing of circ_ZFR inhibited PTX resistance and malignant cell behaviors in PTX-resistant NSCLC cells. Si-circ_ZFR or si-NC was transfected into A549/PTX and H460/PTX cells. a QRT-PCR assay was conducted for circ_ZFR and ZFR expression levels in A549/PTX and H460/PTX cells. b MTT assay was adopted to determine IC50 of PTX in A549/PTX and H460/PTX cells. c, d Flow cytometry analysis was performed to analyze cell cycle process in A549/PTX and H460/PTX cells. e, f MTT assay was utilized for the detection of cell proliferation in A549/PTX and H460/PTX cells. g Western blot assay was conducted for Ki67 protein level in A549/PTX and H460/PTX cells. h Flow cytometry analysis was used for cell apoptosis ability in A549/PTX and H460/PTX cells. i, j Transwell assay was conducted to examine the migration and invasion of A549/PTX and H460/PTX cells. k, l Western blot assay was conducted for the protein levels of Twist1, E-cadherin and N-cadherin in A549/PTX and H460/PTX cells. *P < 0.05