Fig. 4
Circ_ZFR promoted PTX resistance and cell progression in PTX-resistant NSCLC cells by targeting miR-195-5p. A549/PTX and H460/PTX cells were treated with si-NC, si-circ_ZFR, si-circ_ZFR + anti-miR-NC or si-circ_ZFR + anti-miR-195-5p. a QRT-PCR assay was utilized for miR-195-5p level in A549/PTX and H460/PTX cells. b IC50 of PTX in A549/PTX and H460/PTX cells was estimated by MTT assay. c–f Cell cycle and cell proliferation in A549/PTX and H460/PTX cells were analyzed by flow cytometry analysis and MTT assay, respectively. g Western blot assay was conducted for Ki67 protein level in A549/PTX and H460/PTX cells. h Cell apoptosis, i, j migration and invasion in A549/PTX and H460/PTX cells were assessed by flow cytometry analysis and transwell assay, respectively. k, l The protein levels of Twist1, E-cadherin and N-cadherin in A549/PTX and H460/PTX cells were measured through western blot assay. *P < 0.05