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Fig. 5 | Cancer Cell International

Fig. 5

From: Circ_ZFR contributes to the paclitaxel resistance and progression of non-small cell lung cancer by upregulating KPNA4 through sponging miR-195-5p

Fig. 5

KPNA4 was target gene of miR-195-5p. a The predicted binding sites between KPNA4 3′ UTR and miR-195-5p. b, c The luciferase activity in A549/PTX and H460/PTX cells co-transfected with miR-195-5p/miR-NC and KPNA4-wt/KPNA4-mut was measured by dual-luciferase reporter assay. df The levels of miR-195-5p, KPNA4 mRNA and KPNA4 protein in A549/PTX and H460/PTX cells transfected with miR-195-5p, miR-NC, anti-miR-195-5p or anti-miR-NC were measured by qRT-PCR assay or western blot assay. g, h The mRNA and protein levels of KPNA4 in HBE, A549, H460, A549/PTX and H460/PTX cells were determined using qRT-PCR assay and western blot assay, respectively. i The mRNA level of KPNA4 in normal tissues, PTX-sensitive and PTX-resistant NSCLC tissues was determined by qRT-PCR assay. j, k The correlation between miR-195-5p and KPNA4 mRNA in PTX-sensitive and PTX-resistant tumor tissues was analyzed by spearman’s correlation coefficient analysis. l The protein level of KPNA4 in normal tissues, PTX-sensitive and PTX-resistant NSCLC tissues was measured using western blot assay. *P < 0.05

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