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Fig. 6 | Cancer Cell International

Fig. 6

From: Circ_ZFR contributes to the paclitaxel resistance and progression of non-small cell lung cancer by upregulating KPNA4 through sponging miR-195-5p

Fig. 6

MiR-195-5p overexpression suppressed PTX resistance and malignant behaviors of PTX-resistant NSCLC cells by binding to KPNA4. A549/PTX and H460/PTX cells were assigned to miR-195-5p, miR-NC, miR-195-5p + KPNA4 and miR-195-5p + pcDNA groups. a, b The mRNA and protein levels of KPNA4 in A549/PTX and H460/PTX cells were determined by qRT-PCR assay and western blot assay, respectively. c IC50 of PTX in A549/PTX and H460/PTX cells was evaluated using MTT assay. dg Cell cycle and cell proliferation in A549/PTX and H460/PTX cells were estimated using flow cytometry analysis and MTT assay, respectively. h The protein level of Ki67 in A549/PTX and H460/PTX cells was measured using western blot assay. i Cell apoptosis, j, k migration and invasion in A549/PTX and H460/PTX cells were analyzed by flow cytometry analysis and transwell assay, respectively. l, m The protein levels of Twist1, E-cadherin and N-cadherin in A549/PTX and H460/PTX cells were measured by western blot assay. *P < 0.05

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