Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: LncRNA LINC00342 contributes to the growth and metastasis of colorectal cancer via targeting miR-19a-3p/NPEPL1 axis

Fig. 3

MiR-19a-3p is downregulated in CRC and negatively regulated by LINC00342. Expression of miR-19a-3p in CRC tumor tissues (a) and cell lines (b); **P < 0.01 vs. the non-tumor group or NCM460 cells. The miRNA target sites of LINC00342 was predicted by using DIANA tools, the miR-19a-3p binding site and the mutational LINC00342 sequence were shown (c); expression of miR-19a-3p in SW480 and HT-29 cells transfected with miR-19a-3p mimic or mimic NC (d); SW480 and HT-29 cells were co-transfected with the wild or mutant type LINC00342 plasmids and miR-19a-3p mimic or mimic NC, and the luciferase activities were measured (e); **P < 0.01 vs. NC mimic group. RIP assays were performed in SW480 and HT-29 cells, and precipitated RNA levels were presented as fold changes in Ago2 relative to IgG immunoprecipitates (f); **P < 0.01 vs. IgG group. Expression of miR-19a-3p in SW480 and HT-29 cells transfected with sh-NC or sh-LINC00342 (g); **P < 0.01 vs. sh-NC group. Expression of LINC00342 in SW480 and HT-29 cells transfected with NC-mimic or miR-19a-3p mimic (h); **P < 0.01 vs. NC mimic group

Back to article page