Fig. 5

MiR-532-3p was the target miRNA of LINC00963. a StarBase and b, c dual-luciferase reporter gene assay were used to predict and verify that miR-532-3p binds to LINC00963. (D-E) RNA Immunoprecipitation was used to further verify that miR-532-3p binds to LINC00963. f The expression of miR-532-3p was down-regulated in colon cancer tissues (n = 50), and the results were detected by RT-qPCR. g, h The expression of miR-532-3p was negatively correlated with LINC00963, and the results were analyzed by Pearson correlation coefficient. i, j RT-qPCR was used to detect the expression of miR-532-3p in HCT116 cells or LOVO cells transfected with Blank, siNC, siLINC00963, and LINC00963, respectively. U6 served as a control. The experiment was independently repeated three times. RT-qPCR: real-time quantitative polymerase chain reaction. ^△△△P < 0.001 vs Blank; ^***P < 0.001 vs siNC; ^{^^^}P < 0.001 vs NC; ^&&&P < 0.001 vs Normal