Skip to main content
Fig. 8 | Cancer Cell International

Fig. 8

From: PPP2R2B downregulation is associated with immune evasion and predicts poor clinical outcomes in triple-negative breast cancer

Fig. 8

PPP2R2B downregulation promoted M1 phenotype polarization and migration capability. a Immunofluorescence assay. b Q-PCR assay validated whether the THP-1 monocytes had been successfully polarized into M1 or M2 macrophages. c Q-PCR assay identified that PPP2R2B was significantly upregulated in M1 macrophages compared with M2 macrophages. d Western blotting evaluated the efficacy of PPP2R2B expression. e The schematic diagram of co-culture system. f Cocultured macrophages exhibited different PPP2R2B expression. g Altered expression of polarization-specific genes in macrophages cocultured with TNBC cells transfected with empty or PPP2R2B overexpressing vector, respectively. upper panel: Cocultured with MDA-MB-231 cells; lower panel: Cocultured with MDA-MB-468 cells. h, i Cocultured macrophage was selected to perform the transwell assay. j, k Transwell assay identified that PPP2R2B-educated macrophages could significantly suppress TNBC cells migration. ns (Not significant), *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001

Back to article page