Fig. 5

Wnt/β-catenin pathway inhibition eliminated the promoting effect of PlGF knockdown on proliferation and glycolysis of PC9 cells. a, b The efficiency of β-catenin knockdown in PC9 cells were detected by Q-PCR and western blot. c After treatment of PlGF overexpression combined with β-catenin knockdown, cell proliferation was measured by CCK-8 assay; PC9 cells are transfected with oePlGF or vector, and then are treated with XAV939, specific inhibitor of Wnt/β-catenin pathway, for 24 h. d Measurement of cell proliferation byCCK-8 assay; e Evaluation of OCR level by cell mito stress test assay; f Evaluation of ECAR level by glycolysis stress test assay; g Detection of PlGF, C-myc, LDHA, and β-catenin by Western blot. *p < 0.05, **p < 0.01 vs. Vector; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. oePlGF + Vehicle. oePlGF represents PlGF overexpression. shβ-catenin represents β-catenin knockdown