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Fig. 2 | Cancer Cell International

Fig. 2

From: LncRNA SNHG16 contributes to tumor progression via the miR-302b-3p/SLC2A4 axis in pancreatic adenocarcinoma

Fig. 2

Knockdown of SNHG16 inhibits the proliferation of PC cells and induces their apoptosis. BxPC3 and Panc-1 PC cells were transfected separately with sh-SNHG16 or sh-NC. a Relative expression levels of SNHG16 in PC cells were detected by qRT-PCR. b The viability of PC cells was analyzed by a CCK8 assay. c The proliferation ability of PC cells was assessed by an EdU assay. d The cell proliferation markers PCNA and Ki-67 were detected by western blotting. e The apoptosis rate of PC cells was analyzed by flow cytometry. f Western blot analysis of the apoptosis-related proteins Bax, Bcl-2, cleaved caspase-3 and cleaved caspase-9. β-Actin was used as the internal control. *< 0.05, **P < 0.01, ***P < 0.001

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