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Fig. 4 | Cancer Cell International

Fig. 4

From: LncRNA SNHG16 contributes to tumor progression via the miR-302b-3p/SLC2A4 axis in pancreatic adenocarcinoma

Fig. 4

SNHG16 acts as a sponge to decrease miR-302b-3p expression in PC cells. a qRT-PCR analysis of SNHG16 target miRNAs in HPY-Y5, BxPC3 and Panc-1 cells. b Schematic diagram of the sites for binding between miR-302b-3p and SNHG16 (WT or Mut). c Luciferase activity of SNHG16 WT or Mut in BxPC3 and Panc-1 cells cotransfected with miR-302b-3p mimics or NC mimics. d Relative expression levels of miR-302b-3p in BxPC3 and Panc-1 cells with SNHG16 silencing. e RIP analysis of SNHG16 and miR-302b-3p enrichment in the anti-Ago2 immunoprecipitate in BxPC3 and Panc-1 cells. IgG was used as the control. f, g qRT-PCR assay of miR-302b-3p expression in PC tissues and cell lines. *< 0.05, **P < 0.01, ***P < 0.001

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