Fig. 2
From: Verteporfin disrupts multiple steps of autophagy and regulates p53 to sensitize osteosarcoma cells
Effect of VP or CQ exposure on HOS cells. a Cell viability analysis, measured through MTT assay after exposure to CQ (10 µM). b Cell viability analysis, measured through MTT assay upon exposure to VP. c Cellular uptake after exposure to VP for different time points. d Immunoblot showing expression of total caspase-3 after VP exposure (10 µM) for 24 h. GAPDH is used as a loading control. e Fold change in caspase-3 enzyme activity after VP treatment (10 µM) for 24 h. Enzyme activity in untreated control is taken as '1′. f Gene expression of EMT markers was analyzed using RT-PCR after VP exposure (10 µM) for 24 h. Fold change is plotted taking gene expression of the untreated sample as '1′. ' ∗ ’ indicates a significant difference compared to untreated cells