Fig. 6
From: Verteporfin disrupts multiple steps of autophagy and regulates p53 to sensitize osteosarcoma cells
VP induces HMW protein of P53. Immunoblots showing P53 expression after VP treatment (10 µM) at different doses (a.i.), or (a.ii.) and different time points in HOS cells. (b.i) Immunofluorescence images showing P53 protein upon VP exposure in HOS, and (b.ii) H1299 cells stably transfected with GOF-R273H-P53 cells. Scale bar: 10 μm. Objective: Plan Apochromat 63x/1.40 oil M27. c Immunoblots showing P62 and P53 expression upon VP treatment in H1299 cells stable transfected with EV or GOF-R273H-P53 or WT-P53. d Immunoblots showing P53 expression after VP treatment in MCF-7 and MDAMB-468 cells. e Immunoblots showing P62 and P53 expression upon VP treatment with or without NAC in HOS cells. The ‘third bracket’ indicates the high molecular weight (HMW) band detected