Fig. 7
From: Verteporfin disrupts multiple steps of autophagy and regulates p53 to sensitize osteosarcoma cells
VP plus MG co-treatment targets P53 to lysosomes. Immunoblots showing LC3B-II (a), TFEB (b), P62 (c.i) and P53 (c.ii.) expressions after VP (10 µM) or VP plus MG treatment for 24 h. The ‘third bracket’ represents an HMW band detected. d and e Immunoblots showing LAMP-2A and HSC70 expression after VP or VP plus MG treatment. (f.i) Immunofluorescence image showing P53 (FITC-green) and Lysotracker-Red; (f.ii) or P53 (FITC-green) and LAMP-2A (TRITC-red) post VP and MG treatment. White arrow indicates co-localization. Scale bar: 10 μm. Objective: Plan Apochromat 63x/1.40 oil M27