Fig. 2

MCCC2 promotes the proliferation, migration, and invasion of HCC cells. a The expression of MCCC2 was determined using western blot after transfection. b Western blot analysis was used to detect the relative protein levels of MCCC2, PCNA, c-Myc, N-cadherin, N-cadherin, and MMP9 in the MCCC2 low expression group and control group for SMMC-7721 cell lines. c SMMC-7721 cells were transfected with vector sg-MCCC2 to examine the proliferation of SMMC-7721 cells by CCK-8 assay. d, e The cells were transfected and seeded into six-well plates for 2W. f, g Flow cytometry was performed to determine the cell cycle distribution of the cells that were transfected. The cell cycle distribution is displayed in the bar chart. h, i Wound-healing assay. HCC cells were transfected with sg-MCCC2 and vector. The wound was observed at 0, 24, and 48 h under a light microscope. j, k Transwell assay. Cells transfected with sg-MCCC2 and vector travelled membrane were observed at 48 h. l qrt-PCR was used to detect Glut1, LHDA, and LHDB expression in the MCCC2 low expression and control groups for SMMC-7721 cell lines. m, n Glucose and lactate consumption was detected in the same groups