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Fig. 5 | Cancer Cell International

Fig. 5

From: MCCC2 promotes HCC development by supporting leucine oncogenic function

Fig. 5

Absence of leucine fails to inhibit the biological characteristics of the HCC cells. a The following experiments were carried out in SMMC-7721 cell lines with relatively high MCCC2 expression and SK-Hep1 cell lines with no MCCC2 expression. The same number of cells were plated in 96-well plates and cultured under normal or leucine conditions, and the number and viability of cells were determined every day by CCK-8 assay. b–g Colony-formation, Wound-healing, and Transwell assays were performed with SMMC-7721 and SK-Hep1 cells treated as described above. h Acetyl-CoA levels were assessed with the knockdown of MCCC2 in SMMC-7721 cells

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