Fig. 5From: miR-636 inhibits EMT, cell proliferation and cell cycle of ovarian cancer by directly targeting transcription factor Gli2 involved in Hedgehog pathwayRescue experiments confirm that miR-636 inhibits cell proliferation and EMT by regulating Gli2. a, b Relative levels of Gli2 mRNA and protein expression were detected by qRT-PCR and Western blot after miR-636 mimic and oe-Gli2 were transfected into cancer cells; c Cell viability, d migration, e cell cycle and f the expression of Hh as well as EMT markers were detected by MTT assay, wound healing assay, flow cytometry and Western blot in each treatment group; *p < 0.05, **p < 0.01, ***p < 0.001Back to article page