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Fig. 2 | Cancer Cell International

Fig. 2

From: Downregulation of tumor‐derived exosomal miR-34c induces cancer‐associated fibroblast activation to promote cholangiocarcinoma progress

Fig. 2

Exosomal miR-34c affects activation of fibroblasts. a qRT-PCR analysis of levels of miR-34c in exosomes derived from HIBEC and HuCCT-1 cells. b qRT-PCR analysis of levels of miR-34c in exosomes derived from HuCCT-1 cells treated with or without miR-34c mimics. c-g CCC-HSF-1 cells are incubated with exosomes for 48 h. qRT-PCR analysis of levels of c miR-34c, and d IL-1β, IL-6, and IL-8. e Western blotting analysis of α-SMA and FAP proteins. f Representative images and quantification of cell migration in CCC-HSF-1 cells; Scale bar, 50 μm. g Representative confocal images for immunofluorescence staining indicating delivery of FAM-labeled miR-34c (green) to Dil-labeled CCC-HSF-1 cells (red). Co-localized areas, representing delivery of exosomes, appear in yellow; Scale bar, 10 μm. **P < 0.01 vs. NC-Exo groups

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