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Fig. 1 | Cancer Cell International

Fig. 1

From: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc

Fig. 1

ADAM10, Notch1, Cleaved-Notch1, CD44 and PrPc expression in MCF10A and different breast cancer cell lines. a Western blot assay showed protein expression level of ADAM10, Notch1, Cleaved-Notch1, CD44 and PrPc in human mammary epithelial cell line MCF10A and different breast cancer cell lines MCF7, T-47D, SK-BR-3, MDA-MB-231, MDA-MB-468 and BT-549. GAPDH was used as a loading control. Quantitative analysis of band intensities was conducted in western blot analyses. Data are shown as mean ± SEM of three independent experiments. b QRT-PCR assay showed mRNA expression level of ADAM10, Notch1, CD44 and PrPc in different breast cancer cell lines. GAPDH was used as a loading control. Bars represent the mean of triplicate samples; error bars represent SD. Data are representative of three independent experiments. The significant difference between MCF7 and all the other cell line is indicated by *p < 0.05, **p < 0.01

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