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Fig. 3 | Cancer Cell International

Fig. 3

From: ADAM10 is involved in the oncogenic process and chemo-resistance of triple-negative breast cancer via regulating Notch1 signaling pathway, CD44 and PrPc

Fig. 3

Effects of ADAM10 down-regulation are detected in ADAM10 knockdown MDA-MB-231 cells. Knockdown of ADAM10 expression in MDA-MB-231 cells attenuated the migration (a) and invasion (b) ability. c CCK8 assay was used for detection of proliferation in MDA-MB-231 cells with siADAM10 or siControl. d The 50% inhibitory concentration (IC50) value of paclitaxel and adriamycin were tested in MDA-MB-231 cells with siADAM10 or siControl. e Cell-cycle analysis by flow cytometry using propidium iodide showed significant G1/2 cell-cycle arrest in ADAM10 knockdown MDA-MB-231 cells compared to siControl. f The apoptotic cells were detected by PE Annexin V and 7-AAD. Cells that are considered viable are PE Annexin V negative and 7-AAD negative; cells in early apoptosis are PE Annexin V positive and 7-AAD negative; and cells in late apoptosis or already dead are both PE Annexin V and 7-AAD positive. Bars represent the mean of triplicate samples; error bars represent SD. Data are representative of three independent experiments. The significant difference between siControl and siADAM10 is indicated by *p < 0.05, **p < 0.01

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