Fig. 4

ADAM10 affects the functions of MDA-MB-231 cells via regulating Notch signaling pathway, CD44 and PrPc. a Western blot assay showed Notch1, Cleave-Notch1 and its activated targets Cyclin D3, p21Waf1/Cip1, HES1 and c-Myc expression in MDA-MB-231 cells with siADAM10 or siControl for 96 h. GAPDH was used as a loading control. b WB lane stands for lysates from MDA-MB-231 cells immunoblotted with corresponding antibodies without IgG, ADAM10, CD44 or PrPc antibody incubation. IgG lane stands for lysates immunoblotted with corresponding antibodies after IgG incubation. IP lane stands for lysates immunoprecipitated with ADAM10, CD44 or PrPc antibody and immunoblotted with corresponding antibodies. c Cytoplasmic and nuclear extracts from MDA-MB-231 cells with siADAM10 or siControl were tested by Western blot assay, showing protein expression level of ADAM10, CD44 and PrPc. GAPDH was used as a cytoplasmic control and TBP as a nuclear control. Quantitative analysis of band intensities was conducted in western blot analyses. Data are shown as mean ± SEM of three independent experiments