Fig. 5From: Synergism between the phosphatidylinositol 3-kinase p110β isoform inhibitor AZD6482 and the mixed lineage kinase 3 inhibitor URMC-099 on the blockade of glioblastoma cell motility and focal adhesion formationCombined inhibition of PI3Kβ and MLK3 suppressed cell migration and invasion, and blocked the formation of lamellipodia and focal adhesions. a, b Boyden chamber migration assay in U-87 MG and U-118 MG cells treated with AZD6482 and URMC-099 for 6 h. c, d Boyden chamber invasion assay in U-87 MG and U-118 MG cells treated with AZD6482 and URMC-099 for 24 h. P values were determined by One-way ANOVA and Post Hoc multiple comparison Tukey HSD test. Compared with DMSO, *: p < 0.05; ***: p < 0.001; compared with AZD6482, a: p < 0.05; compared with URMC-099, b: p < 0.05. e Immunofluoresescence of U-118 MG cells after treatment with AZD6482 (30 μM) and URMC-099 (3 μM) for 3 h. Representative photographs show the effects of AZD6482 and URMC-099 on the formation of lamellipodia protrusions (white arrows) and FAs (yellow arrows). Bar = 20 μm. f Number of U-118 MG cells with lamellipodia was decreased by the combination of the combination of AZD6482 and URMC-099. g Number of FAs per U-118 MG cell was reduced by the combination of the combination of AZD6482 and URMC-099. P values were determined by One-way ANOVA and Post Hoc multiple comparison Tukey HSD test. *: p < 0.05; **: p < 0.01; ***: p < 0.001. (H) Immunoblotting of U-118 MG cells treated with AZD6482 (30 μM) and URMC-099 (3 μM) alone or in combination for 24 h. Expression of GAPDH was served as a loading control. Data were representative of two independent experimentsBack to article page