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Fig. 3 | Cancer Cell International

Fig. 3

From: Hypoxia-induced Tie1 drives stemness and cisplatin resistance in non-small cell lung carcinoma cells

Fig. 3

Tie1 knockdown enhances the anti-tumor effect of cisplatin in vivo. a A549 cells transfected by scramble or Tie1-shRNA lentiviruses were subcutaneously injected into the right flank of nude mice. Representative images of xenografts at 35 days post-treatment and tumor growth curves delineated on the basis of volume measured every 5 days. *P < 0.05, **P < 0.01, ***P < 0.001 vs sh-Scr + Saline, #P < 0.05, ###P < 0.001 vs sh-Scr + Cisplatin, using two-way ANOVA followed by Bonferroni post hoc test. (n = 6). b Tumor weights of xenografts were evaluated. *P < 0.05, **P < 0.01 vs sh-Scr + Saline, #P < 0.05 vs sh-Scr + Cisplatin. (n = 6). c Expression levels of stemness-related genes were determined by western blot analysis. Relative protein levels quantified by ImageJ and normalized to β-actin. *P < 0.05, **P < 0.01. (n = 3). d Representative images of H&E staining and immunohistochemical staining of Ki67 expression in xenograft tumor tissues. Scale bar = 50 μm. Relative quantification of the IHC staining was performed using ImageJ software. *P < 0.05. (n = 3). e Images of tumor formation in the limiting dilution tumorigenicity assay. Nude mouse was injected with different concentrations of cells subcutaneously in the flank (n = 5). The volume of the tumors was recorded after 5 weeks

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