Fig. 6

HIF-1α controls the stemness of NSCLC cells by regulating Tie1 expression. a Tie1 expression in A549 cells that express HIF-1α vector or HIF-1α and Tie1 shRNA vectors. Relative protein levels quantified by ImageJ and normalized to β-actin. **P < 0.01, ***P < 0.001. (n = 3). b Representative image of spheres in A549 cells that express a HIF-1α vector or HIF1α and Tie1 shRNA vectors. c The expression levels of stemness-related genes were determined by western blot analysis. Relative protein levels quantified by ImageJ and normalized to β-actin. *P < 0.05, **P < 0.01. (n = 3). d The migration ability of A549 cells was evaluated by Transwell assays. The number of migrated cells is shown in the bar graph. *P < 0.05, **P < 0.01. (n = 3)