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Fig. 3 | Cancer Cell International

Fig. 3

From: Circ-CCDC66 upregulates REXO1 expression to aggravate cervical cancer progression via restraining miR-452-5p

Fig. 3

Circ-CCDC66 acts as a molecular sponge for miR-452-5p. Putative downstream miRNAs of circ-CCDC66 were predicted by Miranda (http://miranda.org.uk/) database, four miRNAs were selected for our further study. a Full length of circ-CCDC66 were biotinylated, and the complexes were subjected to qRT-PCR assays. Relative miRNAs expression were presented. b AGO-2 RIP assays were conducted, and the expression of circ-CCDC66 and miR-452-5p in protein bonds were detected by qRT-PCR. c The binding sites between circ-CCDC66 and miR-452-5p were showed. d Luciferase reporter assays were used to determine the interaction between circ-CCDC66 and miR-452-5p in 293 T and Hela cells. e The expression of miR-452-5p in Sh-NC or Sh-circCCDC66#1 transfected Hela and SiHa cells were detected by qRT-PCR. f the expression of miR-452-5p in cervical cancer tissues were measured by qRT-PCR. g Spearman analysis was employed to assess the correlation expression between circ-CCDC66 and miR-452-5p. Data were presented as mean ± SD, all experiments were performed at least three times. **P < 0.01

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