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Fig. 4 | Cancer Cell International

Fig. 4

From: Circ-CCDC66 upregulates REXO1 expression to aggravate cervical cancer progression via restraining miR-452-5p

Fig. 4

REXO1 is a downstream target for miR-452-5p. Potential mRNA targets of miR-452-5p were predicted by PITA (https://genie.weizmann.ac.il/pubs/mir07/mir07_exe.html), RNA22V2 (https://cm.jefferson.edu/rna22/), miRmap (https://mirmap.ezlab.org/downloads/mirmap201301e/), TargetScan Human 7.2 (http://www.targetscan.org/vert_72/) dataset. a Bio-miR-452-5p and Bio-NC probes were constructed, and qRT-PCR was applied to analyze the expression of REXO1 mRNA in the purified bonds. b the binding sites between miR-452-5p and REXO1, mutant sequence of REXO1 was synthesized. c and d Luciferase reporter assays were performed to determine the correlation between miR-452-5p and REXO1 in 293 T and Hela cells. e Hela and SiHa cells were stably transfected with NC mimic, miR-452-5p mimic, miR-452-5p mimic with OE-NC, and miR-452-5p mimic with OE-circ-CCDC66 as indicated, the protein and mRNA levels of REXO1 were measured by qRT-PCR and western blot. f The expression of REXO1 mRNA in cervical cancer tissues were evaluated by qRT-PCR. g The protein expression in randomly selected seven pairs of cervical cancer human samples were detected by western blot. h The expression of miR-452-5p and REXO1 in tumor tissues were analyzed by spearman analysis. Data were presented as mean ± SD, all experiments were performed at least three times. *P < 0.05, **P < 0.01

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