Fig. 5
From: Co-expression of CMTM6 and PD-L1: a novel prognostic indicator of gastric cancer
Immunofluorescence, Cell fractionation assays, Co-immunoprecipitation, Western blot, and small interfering RNA (siRNA) transfection analyses for gastric cancer cell lines. a Immunofluorescence analysis of gastric cancer tissues for CMTM6. Red indicates CMTM6, blue indicates the nuclei. b Immunofluorescence analysis of gastric cancer tissues for PD-L1. Green indicates PD-L1, blue indicates the nuclei. c Immunofluorescence analysis of gastric cancer tissues for CMTM6 and PD-L1. Red indicates CMTM6, green indicates PD-L1, blue indicates the nuclei. d Nuclear and cytoplasm distribution of CMTM6 and PD-L1. e Western blot analysis for gastric cancer cell lines. f, g Knockdown of CMTM6 in SGC-7901 and MGC-803 cells. h co-IP of CMTM6 and PD-L1