Fig. 2From: Ceramide kinase mediates intrinsic resistance and inferior response to chemotherapy in triple‐negative breast cancer by upregulating Ras/ERK and PI3K/Akt pathways CERK overexpression promotes TNBC cell growth and migration. a Western blot image of CERK in TNBC cells after transfecting cells with pCMV3 and pCERK. Overexpression of CERK increases CERK level (b) and proliferation (c) in TNBC cell lines: Hs 578T, MDA-MB-231, and BT-549. d Representative images of Boyden chamber assay showing migrated MDA-MB-231 cells overexpressing CERK. e Quantification shows a significant increase in the number of migrated cells in TNBC cell lines after CERK overexpression. Proliferation and migration were measured after 72- and 24-h incubation; *P < 0.05, compared with pCMV3Back to article page