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Fig. 2 | Cancer Cell International

Fig. 2

From: CircNEIL3 promotes cervical cancer cell proliferation by adsorbing miR-137 and upregulating KLF12

Fig. 2

The features of circNEIL3 in cervical cancer. a The exonic information of circNEIL3 was illustrated as indicated. b qRT-PCR assay with convergent or divergent primers indicating the existence of circNEIL3 in SiHa cells. c The expressions of circNEIL3 and linear mRNA in SiHa cells were detected by qRT-PCR in the presence or absence of RNase R. d qRT–PCR analysis for the expression of circNEIL3 and NEIL3 mRNAs after treatment with Actinomycin D in SiHa cells. e Nuclear-cytoplasmic fractionation experiment showing that circNEIL3 was mainly distributed in the cytoplasm. f FISH for circNEIL3. Nuclei were stained with DAPI. Data were represented as means ± S.D. of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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