Fig. 4

CircNEIL3 serves as a sponge for miR-137 in cervical cancer. a RIP experiments were performed in SiHa cells, and the co-precipitated miR-137 was subjected to qRT-PCR for circNEIL3. b MiR-137 and circNEIL3 binding sequences and circNEIL3 mutation sequences. c Luciferase activity in SiHa cells co-transfected with luciferase reporter containing wild-type or mutant circNEIL3 sequences and miR-137 mimic or control. d FISH showed that circNEIL3 and miR-137 were co-localized in the cytoplasm of SiHa cells. e After upregulation of circNEIL3 expression in the SiHa and Hela cell lines, the miR-137 expression was detected by qRT-PCR. f After upregulation of miR-137 expression in the SiHa and Hela cell lines, the circNEIL3 expression was detected by qRT-PCR. Data were represented as means ± S.D. of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001