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Fig. 5 | Cancer Cell International

Fig. 5

From: Transcription elongation factor A-like 7, regulated by miR-758-3p inhibits the progression of melanoma through decreasing the expression levels of c-Myc and AKT1

Fig. 5

miR-758-3p negatively modulated TCEAL7 expression. a BioVenn (https://www.biovenn.nl/index.php) was built to find the co-regulator of TCEAL7 from miRanda and miRDB diabetes. b RT-PCR analysis of the expression levels of miR-758-3p in melanoma tissues and nevus tissues (normal control). c RT-PCR was applied to determine miR-758-3p levels in normal melanocyte PIG1 and melanoma cell lines WM-115 and A375. d Kaplan–Meier was used to analyze the prognostic value of miR-758-3p in melanoma patients. e Correlation analysis between the expression levels of TCEAL7 and miR-758-3p in 30 melanoma tissues samples were determined. f RT-PCR analysis of the expression levels of miR-758-3p following cell infection with mimics-miR-758-3p or mimic-NC in WM-115 and A375 cells. g Western blotting analysis of the effect of miR-758-3p on the expression of TCEAL7 protein. h Putative binding sites between miR-758-3p and TCEAL7 3′UTR. i, j Dual-luciferase gene reporter system was used to assess the effect of miR-758-3p on the transcriptional activity of TCEAL7. (*P < 0.05, **P < 0.01, ***P < 0.001)

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