Fig. 2

CTCF represses the transcription of miR-137. a qRT-PCR and western blot assays were performed to determine the level of CTCF mRNA and protein. b The expression of miR-137 was examined after CTCF overexpression. c ChIP assays were conducted to investigate the affinity of CTCF in miR-137 promoter (left). Luciferase reporter was conducted to assess the effect of CTCF on the transcriptional activity of miR-137 in HEK-293 T cell (right). d Potential binding site between CTCF and miR-137 promoter region. e The promoter region of miR-137 was divided into five segments and the sequence of F5 was mutated. f ChIP assay was performed to explore the specific fragment in miR-137 promoter responsible for the binding with CTCF. g Luciferase reporter was conducted to determine which sequence in F5 fragment could bind to CTCF. *P < 0.05, ***P < 0.01