Fig. 4
From: CTCF-silenced miR-137 contributes to EMT and radioresistance in esophageal squamous cell carcinoma
CTCF induces DNA methylation of miR-137 via recruiting PRC2 complex. a The potential transcription factor for miR-137 was predicted by UCSC tool. b qRT-PCR and western blot assays were performed to determine the level of Suz12 mRNA and protein as well as miR-137 expression after Suz12 overexpression. c qRT-PCR and western blot assays were performed to determine the level of EZH2 mRNA and protein as well as miR-137 expression after EZH2 overexpression. d Immunofluorescence assay was performed to determine the co-localization of CTCF, Suz12 and EZH2. e ChIP assay was conducted to test the affinity of CTCF, SUZ12, EZH2 and H3K27Me3 to miR-137 promoter. f, g qRT-PCR and western blot assays were performed to determine the level of Suz12 and EZH2 mRNA and protein in CTCF-abundant cells. h ChIP data indicated that Suz12 and EZH2 were recruited by CTCF to enhance the H3K27Me3 level of miR-137. **P < 0.01