Fig. 5
From: CTCF-silenced miR-137 contributes to EMT and radioresistance in esophageal squamous cell carcinoma
MiR-137 inhibits ESCC cell proliferation, migration and radioresistance by targeting EZH2 and PXN. a qRT-PCR analysis was utilized to determine the overexpression of PXN. b, c The level of PXN and EZH2 mRNA and protein were measured by qRT-PCR and western blot assays in indicated cells. d EdU assay was conducted to measure the effects of PXN or EZH2 on miR-137 mimics-mediated cell proliferation. e Transwell assays were performed to detect the effects of PXN or EZH2 on miR-137 mimics-mediated cell migration and invasion. f Western blot assays were conducted to measure the level of EMT-related proteins in indicated cells. g Clonogenic survival assay was performed to testify the effects of PXN or EZH2 on miR-137 mimics-mediated radiosensitivity. h Flow cytometry analysis was performed to evaluate cell apoptosis rate after indicated transfections. *P < 0.05, **P < 0.01