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Fig. 2 | Cancer Cell International

Fig. 2

From: Long non‐coding RNA VPS9D1-AS1 facilitates cell proliferation, migration and stemness in hepatocellular carcinoma

Fig. 2

SEC61A1 is a downstream target of miR-491-5p. a The venn diagram revealed 9 miRNAs which targeted SEC61A1 based on prediction from 2 databases (microT and miRanda). b RNA pull down assay disclosed enrichment of 9 candidate miRNAs pulled down by biotin-labeled SEC61A1. c qRT-PCR examined expression of miR-491-5p and miR-193a-3p in HCC cells and normal liver epithelial cells. d qRT-PCR detected miR-491-5p and SEC61A1 expression under miR-491-5p overexpression. e “ENCORI” database predicted the binding sequences of SEC61A1 and miR-491-5p. f Luciferase reporter assay revealed the luciferase activity of wild and mutant SEC61A1 in response to miR-491-5p up-regulation. *P < 0.05, **P < 0.01. n.s. indicated difference was not statistically significant

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