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Fig. 3 | Cancer Cell International

Fig. 3

From: Long non‐coding RNA VPS9D1-AS1 facilitates cell proliferation, migration and stemness in hepatocellular carcinoma

Fig. 3

VPS9D1-AS1 serves as a sponge of miR-491-5p to up-regulate SEC61A1. a RNA pull down assay demonstrated enrichment of 6 candidate lncRNAs pulled down by biotin-labeled miR-491-5p. b Nucleus/cytoplasm fraction assay revealed subcellular location of VPS9D1-AS1. c Binding sites between VPS9D1-AS1 and miR-491-5p were predicted from “ENCORI” database. d Luciferase reporter assay revealed the luciferase activity of wild and mutant VPS9D1-AS1 in cells co-transfected with miR-491-5p mimics or NC mimics. e Relative expression of VPS9D1-AS1 in HCC cells and normal liver epithelial cells was evaluated by qRT-PCR. f Depletion efficiency of VPS9D1-AS1 in HCC cells and relative SEC61A1 expression were revealed in qRT-PCR. g RIP assay detected enrichment of VPS9D1-AS1, miR-491-5p and SEC61A1 pulled down by anti-AGO2 and anti-IgG. *P < 0.05, **P < 0.01

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