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Fig.2 | Cancer Cell International

Fig.2

From: Suppression of TCAB1 expression induced cellular senescence by lessening proteasomal degradation of p21 in cancer cells

Fig.2

Knockdown of TCAB1 inhibited the degradation of p21 in HeLa cells. a HeLa cells infected with shTCAB1 lentivirus following DMSO (NC) or MG132 (20 μM) treatments for 6 h. The protein levels of TCAB1 and p21 were detected by western blotting. b The shTCAB1 transformed HeLa cells from lentiviral infections were treated with MG132 (20 μM) for 6 h. Cell Lysates were immunoprecipitated with anti-p21 antibody and analyzed by western blotting using an anti-ubiquitin antibody. c The protein levels of TCAB1, p53, p21and p16 were determined by Western blotting in shTCAB1 lentivirus infected cells and the control cells. d The mRNA expression levels of p21 were measured by qRT-PCR in HeLa infected with shTCAB1 lentivirus after treated with DMSO (NC) or MG132 (20 μM)

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Cancer Cell International

ISSN: 1475-2867

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