Fig.4

Depletion of p21 in TCAB1 knockdown cells attenuated the development of senescence phenotype. a To determine the half-life of p21, A549 cells were treated with cycloheximide (100 μg/ml) for indicated periods of time, and then immunoblotted with probing antibodies. b Quantification of the p21 protein levels relative to β-actin. Intensities of blots were analysed by the ImageJ software. c SA-β-gal staining in shTCAB stable A549 lung cancer cells transfected with either a scramble control or p21 siRNA. d Quantification from C for SA-β-gal positive cells in percentages (n = 8). e The protein levels of TCAB1 and p21 determined by western blotting