Fig. 1

miR-17-5p is upregulated in LSCC tissues and LSCC cell lines. a Hematoxylin and eosin staining of normal laryngeal tissues and LSCC tissues. b qRT-PCR was used to detected miR-17-5p expression level in LSCC tissues (n = 39) and adjacent normal tissues (n = 39). Normalized to U6. **P < 0.01 vs. normal tissues. c qRT-PCR was used to detect the miR-17-5p expression level in the tissues from patients with LSCC in T 3/4 stage (n = 16) and the tissues from patients in T 1/2 stage (n = 23). *P < 0.05 vs. T 1/2 stage. d qRT-PCR was used to detect miR-17-5p expression level in tissues from patients with LSCC in TNM I/II stage (n = 15) and in III/IV stage (n = 24). *P < 0.05 vs. TNM I/II stage. e Kaplan–Meier analysis was used to analyze the survival rate of LSCC patients with low or high miR-17-5p level. f qRT-PCR was used to detect miR-17-5p expression level in three LSCC cell lines (SCC-2, SCC-40 and Hep2) compared with human oral keratinocyte cell line HOK. *P < 0.05, **P < 0.01 vs. HOK cell