Fig. 3

Depletion of miR-17-5p suppresses AKT phosphorylation. a Hep2 cells and SCC-2 cells were transfected with miR-17-5p inhibitor or inhibitor-NC. Western blot analysis was used to detect the protein levels of BCL-2, BAX, cleaved Caspase-3. Right panel shows densitometric analysis. *P < 0.05, **P < 0.01 vs. inhibitor-NC. b Cells were treated as (a), mRNA levels of BCL-2 and BAX were detected by using qRT-PCR. Normalized against β-actin. **P < 0.01, ***P < 0.001 vs. inhibitor-NC. c Cells were treated as (a), protein levels of p-AKT and AKT were detected by western blot analysis. Right panel shows densitometric analysis. **P < 0.01 vs. inhibitor-NC. d Cells were treated as (a), PTEN protein level was detected by western blot analysis. Right panel shows densitometric analysis