Fig. 5

PIK3R1 is downregulated in LSCC tissues and cell lines. a qRT-PCR was used to detect the mRNA level of PIK3R1 in LSCC tissues (n = 39) and adjacent normal tissues (n = 39). *P < 0.05 vs. normal tissues. b Western blot analysis was used to detect the protein level of PIK3R1 in LSCC tissues and adjacent normal tissues. The representative experiments were present. c qRT-PCR was used to detect PIK3R1 mRNA expression in three LSCC cell lines (SCC-2, SCC-40 and Hep2) compared with human oral keratinocyte cell line HOK. *P < 0.05, **P < 0.01 vs. HOK cell. d Western blot analysis was used to detect PIK3R1 protein level in three LSCC cell lines (SCC-2, SCC-40 and Hep2) compared with human oral keratinocyte cell line HOK. The representative experiments were present. e Pearson correlation was used to analyze the relationship between miR-17-5p and PIK3R1 mRNA (R = − 0.4852, P = 0.0100)