Fig. 6

miR-17-5p/PIK3R1 axis plays an essential role in LSCC cell proliferation. a Hep2 cells were transfected with pcDNA3.1-PIK3R1 or empty vector. The mRNA level of PIK3R1 was detected by qRT-PCR. ***P < 0.001 vs. empty vector. b Hep2 cells were treated as (a), the protein level of PIK3R1 was detected by western blot analysis. **P < 0.01 vs. empty vector. c Hep2 cells were transfected with pcDNA3.1-PIK3R1 and miR-17-5p inhibitor respectively or transfected them together. Cell apoptosis was detected by Annexin V-FITC/PI staining. Below panel shows the analysis of apoptosis rate. **P < 0.01, ***P < 0.001 vs. corresponding negative control; ^#P < 0.05 vs. pcDNA3.1-PIK3R1. d Hep2 cells were treated as (c), cell viability was detected by CCK-8 assay in different times. **P < 0.01 vs. corresponding negative control; ^#P < 0.05 vs. pcDNA3.1-PIK3R1. e Hep2 cells were treated as (c), the protein levels of PIK3R1, BCL-2, BAX, cleaved Caspase-3, p-AKT and AKT were detected by western blot analysis. Below panel shows densitometric analysis. *P < 0.05, **P < 0.01 vs. corresponding negative control; ^#P < 0.05 vs. pcDNA3.1-PIK3R1