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Fig. 7 | Cancer Cell International

Fig. 7

From: Silencing of miR-17-5p suppresses cell proliferation and promotes cell apoptosis by directly targeting PIK3R1 in laryngeal squamous cell carcinoma

Fig. 7

miR-17-5p is involved in LSCC proliferation in vivo. Hep2 cells engineered to stably knockdown of miR-17-5p (LV-anti-miR-17-5p) or negative control (LV-anti-miR-NC). Then cells were injected subcutaneously in 200 μl 1640/Matrigel (100:100) into the left posterior ankle of the nude mice to establish xenograft tumors (each group n = 6). a Images of tumors excised from each group of mice at the final time point (28 days after injection) and the hematoxylin and eosin staining of xenograft tumor. b Tumor volumes were monitored by direct measurement with calipers and calculated by the formula: (length × width2)/2. *P < 0.05, **P < 0.01 vs. LV-ant-miR-NC. c Average weight of tumors in nude mice. *P < 0.05 vs. LV-anti-miR-NC. d RNA was extracted from excised tumors and the mRNA level of PIK3R1 was detected by using qRT-PCR. **P < 0.01 vs. LV-anti-miR-NC. e Total proteins were extracted from excised tumors and the protein levels of PIK3R1, BCL-2, BAX and cleaved Caspase-3 protein were determined by western blotting. Right panel shows densitometric analysis. *P < 0.05, **P < 0.01 vs. LV-anti-miR-NC

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